Journal: Microarrays

Article Title: Development and Optimization of a Thrombin Sandwich Aptamer Microarray

doi: 10.3390/microarrays1020095

Figure Lengend Snippet: Specificity of aptasensor for thrombin. Fluorescence signal (635 nm) recorded on thrombin aptamer microarray after the incubation of the different indicated proteins with TBA2-Cy5 (protein and TBA2 final concentration: 500 nM and 1 mM, respectively); ( a ) thrombin, blank (TBA2-Cy5 only), BSA, lysozyme and VEGF and ( b ) corresponding microarray images.

Article Snippet: TBA1 aptamers were loaded into microarray plates and submitted to the Microarray Spotter (Versarray Chip Writer Pro System, BioRad) for slide printing, using Telechem SMP3 microspotting pins (Arrayit, Sunnyvale, CA, USA).

Techniques: Fluorescence, Microarray, Incubation, Concentration Assay

Journal: Microarrays

Article Title: Development and Optimization of a Thrombin Sandwich Aptamer Microarray

doi: 10.3390/microarrays1020095

Figure Lengend Snippet: Effect of incubation time. Fluorescence signal (635 nm) recorded on TBA1 microarray ( a ) after the incubation of thrombin and TBA2-Cy5 for different times (180–120–90–60–30–15–5 min) and ( b ) corresponding microarray images.

Article Snippet: TBA1 aptamers were loaded into microarray plates and submitted to the Microarray Spotter (Versarray Chip Writer Pro System, BioRad) for slide printing, using Telechem SMP3 microspotting pins (Arrayit, Sunnyvale, CA, USA).

Techniques: Incubation, Fluorescence, Microarray

Journal: Microarrays

Article Title: Development and Optimization of a Thrombin Sandwich Aptamer Microarray

doi: 10.3390/microarrays1020095

Figure Lengend Snippet: Effect of serum. Fluorescence signal (635 nm) recorded on TBA1 microarray ( a ) after the incubation of thrombin and TBA2-Cy5 complexes in presence of different Fetal Bovine Serum (FBS) dilutions (0%–10%–20%–30%–40%–50%–65%) and ( b ) corresponding microarray images.

Article Snippet: TBA1 aptamers were loaded into microarray plates and submitted to the Microarray Spotter (Versarray Chip Writer Pro System, BioRad) for slide printing, using Telechem SMP3 microspotting pins (Arrayit, Sunnyvale, CA, USA).

Techniques: Fluorescence, Microarray, Incubation

Journal: Microarrays

Article Title: Development and Optimization of a Thrombin Sandwich Aptamer Microarray

doi: 10.3390/microarrays1020095

Figure Lengend Snippet: Direct and indirect method for thrombin detection. ( a ) Fluorescence signal (635 or 532 nm) plot of thrombin aptamer microarray after the incubation of thrombin and TBA2-Cy5 (direct method, red squares, 635 nm) or thrombin and TBA2-biotin plus Cy3-streptavidin (indirect method, green dots, 532 nm) and ( b ) relative microarray images. Tested thrombin dilutions were: 200 nM–100 nM–50 nM–10 nM–5 nM–1 nM–0 nM. TBA2-Cy5 (red squares) or TBA2-biotin (green dots) concentration was 400 nM. For the indirect method the microarray slide was incubated for 1 h with Cy3-streptavidin, for a total incubation time of two hours in all cases.

Article Snippet: TBA1 aptamers were loaded into microarray plates and submitted to the Microarray Spotter (Versarray Chip Writer Pro System, BioRad) for slide printing, using Telechem SMP3 microspotting pins (Arrayit, Sunnyvale, CA, USA).

Techniques: Fluorescence, Microarray, Incubation, Concentration Assay

Journal: Microarrays

Article Title: Development and Optimization of a Thrombin Sandwich Aptamer Microarray

doi: 10.3390/microarrays1020095

Figure Lengend Snippet: Thrombin calibration curves in FBS solution. ( a ) fluorescence signal (532 nm) plot of TBA1 microarray in 20% FBS solution after the incubation of thrombin and TBA2-biotin plus Cy3-streptavidin and ( b ) corresponding microarray images. Tested thrombin dilutions were: 50 nM–10 nM–5 nM–1 nM–0.5 nM–0 nM, with a fixed TBA-2 biotin concentration of 200 nM.

Article Snippet: TBA1 aptamers were loaded into microarray plates and submitted to the Microarray Spotter (Versarray Chip Writer Pro System, BioRad) for slide printing, using Telechem SMP3 microspotting pins (Arrayit, Sunnyvale, CA, USA).

Techniques: Fluorescence, Microarray, Incubation, Concentration Assay